Composite

Part:BBa_K3670001:Design

Designed by: Luis Arturo Garcia Cruz   Group: iGEM20_RUM-UPRM   (2021-10-20)


algD promoter with luxI and luxR followed by mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 646
    Illegal BglII site found at 1544
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the algD promoter was derived from Pseudomona aureginosa sequence genome, we had to codon-optimize it so that it would be functional in E. coli DH5 alpha. In addition, initially, this device did not have any sort of gene reporters. This was later added to be able to characterize the system.


Source

The algD promoter is derived from the genome of P. aureginosa spp. The sequence was codon-optimized for E. coli DH5alpha.

References